Development of a sampling regime for research with environmental relevance based on variability of transgene expression in plant

Autor: Struzyna-Schulze, Carla; Mikschofsky, Heike; Mönkemeyer, Wenke; Schmidtke, Jörg; Schmidt, Kerstin; Broer, Inge
In:

Development of a sampling regime for research with environmental relevance based on variability of transgene expression in plant

Autor: C
Ergaenzung: doi:10.1038/npre.2010.4443.
Jahr: 2010

Einordung:
Institut: Professur Agrobiotechnologie

Abstract:
Transgenic plants often produce proteins that are unknown in conventional crops. Therefore, it requires a fundamental analysis before approval of a new genetically modified plant is given. Since the EFSA-guidelines for risk assessment on transgenic plants provide only recommendations but no detailed instructions, we wanted to develop a valid test scheme that defines sample size and sampling organ to enable a statically valid and efficient analysis of transgene expression and its variability. The scheme will be based on data obtained for the variability in expression i. of different recombinant proteins in one variety; ii. of different integration sites of one transgene in one variety; iii. of the same recombinant protein between different varieties and between isogenic clones in a variety. The optimization of experimental procedures shall enable the development of cost and time efficient and valid sampling systems that will be used as a part of the Decision Support System (DSS) that is developed by BioOK

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