A fast and sensitive in vivo method to detect influences of transgene encoded proteins on soil microorganisms and composition

Autor: Horn, Patricia; Schlichting, André; Leinweber, Peter; Broer, Inge
In:

Workshop 2009 der Arbeitskreise Somatische Embryogenese und Gentechnik

Autor: Gesellschaft für Pflanzenbiotechnologie e.V.
Ort: Rostock
Jahr: 2009

Einordung:
Institut: Professur Agrobiotechnologie

Abstract:
There is a constant increase of plants that have to be approved by the European Food Safety Association (EFSA). Up to now the influence of the transgenic plants on soil composition is, if necessary, studied by expensive, time- and labour consuming field releases for several years. Additionally, it has been shown, that environmental influences, like weather or soil heterogeneity overlap possible effects of the transgene, therefore relevant transgene specific effects have never been observed.
For identification of possible influences on soil composition controlled, but nature orientated worst case experiments have to be carried out, that allow the selective addition of specific environmental factors. The obtained data must be sufficient to decide whether further field experiments are necessary. Therefore we developed a 3 month requiring fast and efficient in vivo laboratory test system based on composite Vicia hirsuta plants (wild type shoot with transgenic roots) and efficient examination methods, like mass spectrometric “fingerprint” of plant and soil compounds. The Vicia hirsuta system is already established and has shown to be very sensitive for detection of little influences on Rhizobia-Legume-Symbiosis.

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