Soil adaptation of transgenic in vitro carrot plantlets

Autor: Mikschofsky, H.; Mann, G.; Broer, I.
In:

Journal of Agricultural Science

Auflage: 147 (1)
ISBN: ISSN 0021-8596
Seite: 43 - 49
Jahr: 2009

Einordung:
Institut: Professur Agrobiotechnologie

Abstract:
Adapting in vitro transgenic carrots to soil is the most crucial step preceding the field investigation of transgenic carrots. A low proportion of plants, around 0.20, acclimatize to soil (Hardegger & Sturm 1998) and thus prohibit the generation of high-expression carrot lines. In the present paper, a protocol for an efficient soil transfer is presented and the impact of carrot cultivar, soil substrate,tissue culture, and transformation process on transfer process is analysed. Somatic embryo germinants of Daucus carota cv. Rote Riesen 2 and Lobbericher Gelbe Futtermoehre showed a tremendous survival proportion – up to 1.00 – when transferred to their optimal soil substrate: sandy and loamy
soil, with low content of macro and micro elements and a pH of 5.8. By optimizing the conditions
described here, the proportion of soil cclimatized transgenic carrot plants of D. carota Lobbericher
Gelbe Futtermoehre was increased from 0.1 to 0 . 87, and for the cultivar Rote Riesen from 0.09
to 0.67.

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