Electrochemical detection of 0.6% genetically modified maize MON810 in real

Autor: Mix, Maren; Rüger, Julia; Krüger, Simone: Broer, Inge; Flechsig, Gerd-Uwe
In:

Electrochemistry Communications

Autor: Mix, Maren; Rüger, Julia; Krüger, Simone: Broer, Inge; Flechsig, Gerd-Uwe
Auflage: 22
ISBN: doi:10.1016/j.elecom.2012.06.019
Seite: 137 - 140
Jahr: 2012

Einordung:
Institut: Professur Agrobiotechnologie

Abstract:
We demonstrate an approach for the electrochemical detection of genetically modified maize in real maize flour samples by means of square-wave voltammetry (SWV). After labeling the asymmetric PCR-amplified targets with osmium tetroxide bipyridine [OsO4(bipy)], they were hybridized with immobilized oligonucleotide
probes on gold electrodes. We could detect the maize genes ivrp and SSIIb in near isogenic maize. The transgene cryIa/b and the MON810 specific fragment were detected in all transgenic maize samples, down to a content of 0.6% of MON810 in mixed samples. While it was possible to detect all sequences in the samples containing 100% near isogenic or respectively transgenic maize after a hybridization time of less than 10 min,a hybridization time of 30 min was necessary for the detection of the genetic modifications in samples containing only 0.9 to 0.6% of transgenic maize. No significant detection of the transgene cryIa/b or MON810 was possible when only 0.5% of transgenic maize was present in the sample, most likely due to insufficient amplification of the template DNA.

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