Isolation of cyanophycin from tobacco and potato plants with constitutive plastidic cphATe gene expression

Autor: Neubauer, Katja; Hühns, Maja; Klemke, Friederike; Lockau, Wolfgang; Kahmann, Uwe; Pistorius, Elfriede K.; Kragl, Udo; Broer, Inge
In:

Journal of Biotechnology

Bandangabe: 158
Auflage: 1-2
ISBN: ISSN: 0168-1656
Seite: 50 - 58
Jahr: 2012

Einordung:
Institut: Professur Agrobiotechnologie

Abstract:
A chimeric cyanophycin synthetase gene composed of the cphATe coding region from the cyanobacterium Thermosynechococcus elongatus BP-1, the constitutive 35S promoter and the plastid targeting sequence of the integral photosystem II protein PsbY was transferred to
the tobacco variety Petit Havanna SRI and the commercial potato starch production variety
Albatros. The resulting constitutive expression of cyanophycin synthetase leads to polymer
contents in potato leaf chloroplasts of up to 35 mg/g dry weight and in tuber amyloplasts of
up to 9 mg/g dry weight. Both transgenic tobacco and potato were used for the development
of isolation methods applicable for large-scale extraction of the polymer. Two different
procedures were developed which yielded polymer samples of 80 % and 90 % purity,
respectively.

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