First molecular detection of Pneumocystis spp. in red foxes (Vulpes vulpes LINNAEUS, 1758) and raccoon dogs (Nyctereutes procyonoides GRAY, 1834)
Autor: Riebold, D., Lubig, J., Wolf, P., Wolf, C., Russow, K., Loebermann, M., Slevogt, H., Mohr, E., Feldhusen, F., Reisinger, E.C.
In:
Comparative Immunology, Microbiology and Infectious Diseases
Bandangabe: 73 Auflage: 12 Ergaenzung: Article Number: 101531 ISBN: 01479571 DOI: 10.1016/j.cimid.2020.101531 Jahr: 2020
Einordung:
Institut: Professur Tiergesundheit und Tierschutz
Abstract: Fungal organisms of the genus Pneumocystis may cause Pneumocystis pneumonia (PCP) in humans, but also domestic and wild mammals. Almost every animal species hosts its own genetically distinct Pneumocystis species, however information is sparse. In this study, 62 red foxes (Vulpes vulpes) and 37 raccoon dogs (Nyctereutes procyonoides) were collected in North-East Germany. The lung tissues of the animals were analysed by a new designed specific pan-Pneumocystis mtLSU rRNA gene PCR and sequencing. With this PCR, detection and discrimination of all known Pneumocystis spp. in a single step should be possible. This first detection of Pneumocystis spp. in 29/62 (46.8%) red foxes and 29/37 (78.4%) raccoon dogs indicated, that they harbour two dissimilar strains, as seen by specific single nucleotide position changes (SNPs). Nevertheless, five samples with contrary SNPs showed a probable inter-species transmission. Ansprechpartner
Zur Publikation: Mitarbeiter,
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25.02.2021
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