Improving nematode culture techniques and their effects on amino acid profile with considerations on production costs

Autor: Buck, B.H.; Brüggemann, J.; Hundt, M.; Bischoff, A.A.; Grote, B.; Strieben, S.; Hagen, W. 2014
In:

Journal of Applied Ichthyology

ISBN: 0175–8659
Seite: 1 - 9
Jahr: 2014

Einordung:
Institut: Professur Aquakultur und Sea-Ranching

Abstract:
The purpose of this study was to investigate the effectiveness
of 11 different culture media for production of the free-living
nematode Turbatrix aceti. Several other harvesting methods
were tested in addition to mass production. A further focus
was the investigation of amino acid alterations caused by the
application of various media during the culture of T. aceti
and two additional nematode species, Panagrellus redivivus
and Caenorhabditis elegans. Finally, a cost analysis for the
production of T. aceti was generated and its outcome compared
to the production of conventional live feed organisms.
Altogether 11 liquid culture media were tested for mass production
of the nematode Turbatrix aceti using a minimum of
effort in terms of labour and costs. Six harvesting methods,
including filtration as well as active swimming of T. aceti
were evaluated. Additional to the culture of T. aceti in four
of the above-mentioned media, the nematodes P. redivivus
and C. elegans were cultured on two different solid media.
Cost analysis for the production of T. aceti includes those of
the media, the equipment, as well as the labour costs for culture
and harvest. An average density of approx.
30 9 106
8.13 9 106 nematodes L
1 was achieved for
T. aceti. The most efficient method (20 lm filtration) allowed
harvesting 85.3
2.7% of the nematodes from the medium
without disturbing the particles. Lowest efficiency was
achieved by combining sedimentation and filtration, accomplishing
a harvest of 42.1
5.8%. The amino acid profile of
all three nematode species turned out to be both stable and
very similar. Amino acid enrichment had little effect. The
costs for producing one million T. aceti individuals ranged
between 5.39 and 6.19 €, where labour costs accounted for
73 to 84% of the total production costs. In conclusion,
T. aceti appears to be very robust, easy to handle, as well as
cheaper to cultivate compared to other live-feed organisms.
Therefore, its use in commercial aquaculture should be given
future consideration.

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